Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with Acrolein monoclonal antibody (Product # MA5-45767) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.